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Series : Methods in Molecular Biology

Plant Stress Tolerance, Vol. 639, Ed. 1

Methods and Protocols

¥ 11,556 (tax included)

Description

A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical. In Plant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results. Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Plant Stress Tolerance: Methods and Protocols provides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.

Contents

PARTI REVIEWS AND OVERVIEW CHAPTERS

1. Dehydration Tolerance in Plants
Melvin J. Oliver, John C. Cushman, and Karen L. Koster

2. Approaches to Identifying Genes for Salinity Tolerance and the Importance of Timescale
Rana Munns

3. Gene Regulation During Cold Stress Acclimation in Plants
Viswanathan Chinnusamy, Jian-Kang Zhu, and Ramanjulu Sunkar

4. Redox-Dependent Regulation, Redox Control and Oxidative Damage in Plant Cells Subjected to Abiotic Stress
Karl-Josef Dietz

5. Array Platforms and Bioinformatics Tools for the Analysis of Plant Transcriptome in Response to Abiotic Stress
Nese Sreenivasulu, Ramanjulu Sunkar, Ulrich Wobus, and Marc Strickert

6. Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants
Ananda Mustafiz, Khirod K. Sahoo, Sneh L. Singla-Pareek, and Sudhir K. Sopory

PART II GENOME-WIDE APPROACHES FOR IDENTIFICATION OF STRESS-REGULATED GENES, PROTEINS, AND SMALL RNAS

7. Genetic Screens to Identify Plant Stress Genes
Csaba Papdi, Jeffrey Leung, Mary Prathiba Joseph, Imma Perez Salamo, and Laszlo Szabados

8. Arabidopsis Tiling Array Analysis to Identify the Stress-Responsive Genes
Akihiro Matsui, Junko Ishida, Taeko Morosawa, Masanori Okamoto, Jong-Myong Kim, Yukio Kurihara, Makiko Kawashima, Maho Tanaka, Taiko Kim To, Kentaro Nakaminami, Eli Kaminuma, Takaho A. Endo, Yoshiki Mochizuki, Shuji Kawaguchi, Norio Kobayashi, Kazuo Shinozaki, Tetsuro Toyoda, and Motoaki Seki

9. Identification of Stress-Responsive Genes in Plants Using Suppression Subtraction Hybridization: Ozone Stress as an Example
Lila Peal, Michael Puckette, and Ramamurthy Mahalingam

10. Identification of DNA-Binding Proteins and Protein-Protein Interactions by Yeast One-Hybrid and Yeast Two-Hybrid Screen
Peter Klein and Karl-Josef Dietz

11. Functional Characterization of Water-Deficit Stress Responsive Genes Using RNAi
Muthappa Senthil-Kumar, Makarla Udayakumar, and Kirankumar S. Mysore

12. Difference Gel Electrophoresis as a Tool to Discover Stress-Regulated Proteins
Jenny Renaut

13. Thiol- Disulfide Redox Proteomics in Plant Research
Meenakumari Muthuramalingam, Karl-Josef Dietz, and Elke Stroher

14. Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants
Jose Luis Reyes, Catalina Arenas-Huertero, and Ramanjulu Sunkar

15. An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants
Xiaoyun Jia, Venugopal Mendu, and Guiliang Tang

PART III ANTIOXIDANT ENZYMES AND METABOLITES

16. Spectrophotometric Assays for Antioxidant Enzymes in Plants
Sathya Elavarthi and Bjorn Martin

17. Affinity Purification and Determination of Enzymatic Activity of Recombinantly Expressed Aldehyde Dehydrogenases
Hans-Hubert Kirch and Horst Rohrig

18. Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte Leakage in Plants
Niranjani Jambunathan

PART IV OSMOTIC ADJUSTMENT AND ION MEASUREMENTS

19. Quantification of Water Stress-Induced Osmotic Adjustment and Proline Accumulation for Arabidopsis thaliana Molecular Genetic Studies
Paul E. Verslues

20. Methods for Determination of Proline in Plants
Edit Abraham, Cecile Hourton-Cabassa, Laszlo Erdei, and Laszlo Szabados

21. A New Method for Accurately Measuring 1-Pyrroline-5-Carboxylate Synthetase Activity
Elodie Parre, Jacques de Virville, Francoise Cochet, Anne-Sophie Leprince, Luc Richard, Delphine Lefebvre-De Vos, Mohamed Ali Ghars, Marianne Bordenave, Alain Zachowski, and Arnould Savoure

22. Extraction and Analysis of Soluble Carbohydrates
Niels Maness

23. Measuring Soluble Ion Concentrations (Na+, K+, Cl-) in Salt-Treated Plants
Rana Munns, Patricia A. Wallace, Natasha L. Teakle, and Timothy D. Colmer