学術洋書、ソフトウェア、データベースの専門店 Neutrino WebShop

戻る / Previous page

印刷 / Print

• Featured

Culture of Animal Cells, Ed. 6
動物細胞の培養 第6版

A Manual of Basic Technique and Specialized Applications

¥ 7,709 (tax included)

Description

■動物細胞培養の基本書として、高い評価を受けているタイトルの改訂第6版。細胞培養のためのプロトコルを多数提示し、それらをどう応用するかをアドバイスする。


This is the 6th edition of a previously successful book on the basic principles and procedures employed in cell and tissue culture. It also contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture.

* Updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer.
* Presents the current problems, contamination, and misidentification (including cross-contamination) and tries to identify areas of current application with protocols in some of the more popular areas
* Problem-solving chapter at the end, together with a glossary of terms, advice on sourcing materials, and a list of suppliers.


R. IAN FRESHNEY, PhD, is an honorary Senior Research Fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.

Contents

1 Introduction.

2 Biology of Cultured Cells.

3 Laboratory Design, Layout, and Equipment.

4 Equipment and Materials.

5 Aseptic Technique.

6 Safety, Bioethics, and Validation.

7 Culture Vessels and Substrates.

8 Defined Media and Supplements.

9 Serum-Free Media.

10 Preparation and Sterilization.

11 Primary Culture.

12 Subculture and Cell Lines.

13 Cloning and Selection.

14 Cell Separation.

15 Characterization.

16 Differentiation.

17 Transformation and Immortalization.

18 Contamination.

19 Cryopreservation.

20 Quantitation.

21 Cytotoxicity.

22 Specialized Cells.

23 Stem Cells, Germ Cells, and Amniocytes.

24 Culture of Tumor Cells.

25 Three-Dimensional Culture.

26 Scale-up and Automation.

27 Specialized Techniques.

28 Training Programs.

29 Problem Solving.

30 In Conclusion.

Appendix I: Calculations and Preparation of Reagents.

Appendix II: Sources of Equipment and Materials.

Appendix III: Suppliers and Other Resources.

Appendix IV: Glossary.

Appendix V: Cross-contaminated or Misidentified Cell Lines.

Appendix VI: General Textbooks and Relevant Journals.